Journal: Journal of Ovarian Research

Article Title: Augmented inhibition of angiogenesis by combination of HER2 antibody chA21 and trastuzumab in human ovarian carcinoma xenograft

doi: 10.1186/1757-2215-3-20

Figure Lengend Snippet: Tumor microvessel density (MVD) and VEGF expression . (A) The VEGF and CD34 (marker for MVD) expression in SKOV-3 xenografts were detected by immunohistochemistry. (B) Tumor MVD and MOD of VEGF expression were calculated and the values were shown as percents of the control treatment. *, P < 0.01 compared with control. **, P < 0.01 compared with chA21 or trastuzumab alone.

Article Snippet: After antigen retrieval with autoclaving in citric acid, and inactivating endogenous peroxidase with 3% H 2 O 2 , the slides were incubated with the rabbit anti-mouse antibody CD34 (1: 200, Bioss, China) or the rabbit anti-human antibody VEGF (working solution, ZhongShan, China) overnight at 4°C.

Techniques: Expressing, Marker, Immunohistochemistry

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on the growth and angiogenesis of/in melanoma xenografts. ( A ) Melanoma xenograft tumor volumes during the test period; ( B ) melanoma xenograft tumor weight at necropsy; ( C ) immunohistochemical expression of CD34 in melanoma xenografts. Data expressed as mean ± SD; * p < 0.05, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-mouse CD34 polyclonal antibody was purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Immunohistochemical staining, Expressing

Journal: International Journal of Nanomedicine

Article Title: Noninvasive evaluation of the migration effect of transplanted endothelial progenitor cells in ischemic muscle using a multimodal imaging agent

doi: 10.2147/IJN.S152976

Figure Lengend Snippet: Morphological and immunocytochemical analyses of mouse bone marrow-derived EPCs. ( A ) The shape of mononuclear cells was thin and flat on day 3, and a round and fusiform appearance was subsequently observed on day 7 (magnification ×100). ( B ) On day 14, the cells exhibited a typical fusiform or “cobblestone” morphology (magnification ×100). ( C) Uptake of Dil-labeled acLDL and binding of endothelial-specific fluorescein isothiocyanate-labeled lectin in EPCs in culture for 14 days (magnification ×400). Lectin and lipoprotein positivity colocalized in >95% of cells. ( D ) Immunocytochemical images of cells showing positive staining for the CD34, CD133, VEGF receptor 2, CXCR4, vWF, CD146 and CD31 markers. ( E ) Fluorescence image showing the uptake of multimodal imaging of bCD-PLL by EPCs (day 14) with positive rhodamine signals (magnification ×400). Abbreviations: EPC, endothelial progenitor cell; acLDL, acetylated low-density lipoprotein; VEGF, vascular endothelial growth factor; CXCR4, C-X-C chemokine receptor 4; vWF, von Willebrand factor; bCD, bacterial cytosine deaminase; PLL, poly-L-lysine; UEA, Ulex europaeus agglutinin-1; DAPI, 4′,6-diamidino-2-phenylindole; VEGFR2, VEGF receptor 2.

Article Snippet: Frozen sections of ischemic adductor muscles were incubated using 10 μg/mL rabbit monoclonal anti-mouse CD34 (Santa Cruz Biotechnology Inc, Dallas, TX, USA), CD133 (Abnova, Taipei, Taiwan), VEGF receptor 2 (Abcam, Cambridge, UK) and CXCR4 (Abcam) antibodies followed by staining with an Alexa Fluor 488 goat anti-rabbit polyclonal antibody (Thermo Fisher Scientific).

Techniques: Derivative Assay, Labeling, Binding Assay, Staining, Fluorescence, Imaging

Journal: International Journal of Nanomedicine

Article Title: Noninvasive evaluation of the migration effect of transplanted endothelial progenitor cells in ischemic muscle using a multimodal imaging agent

doi: 10.2147/IJN.S152976

Figure Lengend Snippet: Detection of EPCs labeled with multimodal imaging probes in ischemic hindlimbs. ( A ) Cy5.5 and random signals from probe-labeled EPCs were colocalized in the injured tissue stained with fluorescent Alexa488-CD34, CD133, VEGF receptor 2 and CXCR4 (EPC markers). Probe-labeled EPCs positive for EPC markers migrated into microvessel walls 3 days after transplantation. Cy5.5 and EPC antibody positive cells are shown by arrows. The scale bars are 30 μm. ( B ) The numbers of Cy5.5-positive and rhodamine double-positive EPCs per square millimeter within the ischemic region were recorded at 3 days after the induction of injury. ( C ) Western blot showing increased VEGF receptor 2, VEGF, CXCR4 and SDF-1α levels in the ischemic muscle of EPC-treated mice on days 1 and 3 compared with the controls. ( D ) and ( E ) Bottom panel shows the results of quantification of the bands. * P <0.05, *** P <0.001. Abbreviations: EPC, endothelial progenitor cell; VEGF, vascular endothelial growth factor; CXCR4, C-X-C chemokine receptor 4; SDF-1α, stromal cell-derived factor-1alpha; rho, rhodamine; DAPI, 4′,6-diamidino-2-phenylindole; VEGFR2, VEGF receptor 2.

Article Snippet: Frozen sections of ischemic adductor muscles were incubated using 10 μg/mL rabbit monoclonal anti-mouse CD34 (Santa Cruz Biotechnology Inc, Dallas, TX, USA), CD133 (Abnova, Taipei, Taiwan), VEGF receptor 2 (Abcam, Cambridge, UK) and CXCR4 (Abcam) antibodies followed by staining with an Alexa Fluor 488 goat anti-rabbit polyclonal antibody (Thermo Fisher Scientific).

Techniques: Labeling, Imaging, Staining, Transplantation Assay, Western Blot, Derivative Assay

Journal: International Journal of Medical Sciences

Article Title: Activated Hair Follicle Stem Cells and Wnt/β-catenin Signaling Involve in Pathnogenesis of Sebaceous Neoplasms

doi: 10.7150/ijms.8383

Figure Lengend Snippet: Activated HFSCs in the SNs. (A-B) Detection of HFSC markers CD34 and SOX-9 in SGs, YSNs and OSNs. CD34 and SOX-9 were located in the hair bulge region (Arrow) but not in SG, while in YSNs and OSNs, CD34 and SOX-9 were located in the basal cells (Arrow).(C-D) Ki67 and BrdU were detected in both SGs and the basaloid sebocytes in all SNs (Arrow). (E) BrdU and SOX-9 Co-localized in the basal cells of YSNs and OSNs but not in SGs (Yellow arrow). Scale bar: 50μm.

Article Snippet: Primary antibodies were as follows: rabbit anti-human K10 (polyclonal IgG, 1:200, Bioss, China); mouse anti-human AE15 (monoclonal IgG, 1:2; gift); mouse anti-human AE13 (monoclonal IgG, 1:10; gift); rabbit anti-mouse CD34 (polyclonal IgG, 1:200, Boster, China); rabbit anti-human SOX-9 (polyclonal IgG, 1:100, Santa Cruz, USA); goat anti-mouse Ki67 (polyclonal IgG, 1:100, Santa Cruz, USA); mouse anti-BrdU antibody (monoclonal IgG, 1:100, Zhongshan, China); rabbit anti-human Wnt10b (polyclonal IgG, 1:100, Santa Cruz, USA); mouse anti-human β-catenin (monoclonal IgG, 1:100, Santa Cruz, USA).

Techniques: